I thought you meant 'Mobile Safari'. Since you have your audio starting as a timeline action, iOS will not be friendly towards that -- starting media of any kind without user interaction is normally blocked by iOS. Tapping on the ladybug will start audio as expected, since you're not violating Apple's rules. This rule dates back to Tripod & Geocities, when everyone would have a terrible midi file playing in the background of their site. A good workaround for this is to have a 'Start' button or some other interaction after which you can immediately make whatever noise you wish.