The change @dbear is referring to is the new Media Engagement Index in Chrome: If people have already approved audio playback on this site, and have spent some time on it, audio will autoplay. But if this is their first visit, the audio will not play back without any user interaction.
So to get your audio to be able to play in Chrome, you’re going to need to have it play in response to someone clicking something. This can be as simple pausing your main timeline, and then creating a button at time 0 which resumes the main timeline and plays your audio. The audio play action needs to basically happen immediately after a click.